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  Indian J Med Microbiol
 

Figure 2: Clot waveform of normal plasma by monitoring of absorbance. (a) Normal “S” clot reaction curve showing the phases of the clotting reaction, change in absorbance (Y-axis) versus acquisition time (X-axis). Indicates the activated partial thromboplastin time value on X-axis. The upper blue-colored trace shows the changes in absorbance observed during the performance of activated partial thromboplastin time with normal reference plasma. The clot waveform is comprised of five main phases: delay (0-A), baseline (A-B), acceleration (B-C), deceleration (C-D), and end point (D-E). (b) The purple-colored curve is the first derivative of the absorbance corresponding to the coagulation velocity. The light blue-colored biphasic curve is the second derivative of the absorbance corresponding to the coagulation acceleration

Figure 2: Clot waveform of normal plasma by monitoring of absorbance. (a) Normal “S” clot reaction curve showing the phases of the clotting reaction, change in absorbance (Y-axis) versus acquisition time (X-axis). Indicates the activated partial thromboplastin time value on X-axis. The upper blue-colored trace shows the changes in absorbance observed during the performance of activated partial thromboplastin time with normal reference plasma. The clot waveform is comprised of five main phases: delay (0-A), baseline (A-B), acceleration (B-C), deceleration (C-D), and end point (D-E). (b) The purple-colored curve is the first derivative of the absorbance corresponding to the coagulation velocity. The light blue-colored biphasic curve is the second derivative of the absorbance corresponding to the coagulation acceleration